Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Characterization of immune cell subtypes in three commonly used mouse strains reveals gender and strain-specific variations

doi: 10.1038/s41374-018-0137-1

Figure Lengend Snippet: Details of antibodies used in the study

Article Snippet: CD80 , APC , Miltenyi , 16–10A1 , 130–102-584 , 3.75 pL.

Techniques: Concentration Assay

Journal: Life sciences

Article Title: TPX2 influences the regulation of macrophage polarization via the NF-κB pathway in lung adenocarcinoma.

doi: 10.1016/j.lfs.2024.122437

Figure Lengend Snippet: Fig.2 Expression of TPX2 in A549 cells affects macrophage polarization. (A) Detection of macrophage surface markers CD80 and CD163 after cell supernatant induction in A549 group, Vehicle group and TPX2-shRNA group. (B) The analysis percentage of macrophage surface marker CD80, CD163; CD80/CD163 after induction by cell supernatant of the three groups. (C) CD80 and CD163 protein expression in macrophages after induction in the supernatant of the three groups cells. (D) The

Article Snippet: THP-1 cell differentiation into macrophages was induced by culturing for 24 h. The supernatant of each group was then applied for 48 h. The induced cells were digested and washed twice, followed by the addition of PE Anti-Human CD80 (B7-1) (PE-65083, Proteintech, China) and APC Anti-Human CD163 (GHI/61) (APC-65169, Proteintech, China) antibodies and resuspended to flow cytometry (BD Biosciences, USA).

Techniques: Expressing, shRNA, Marker

Journal: Journal of Inflammation (London, England)

Article Title: Tissue-plasminogen activator effects on the phenotype of splenic myeloid cells in acute inflammation

doi: 10.1186/s12950-024-00375-0

Figure Lengend Snippet: References of flow cytometry antibodies used for immunophenotyping

Article Snippet: CD80 , REA983 , APC-Vio770 , 130–116-463 , human IgG1/REA293 , Miltenyi Biotec.

Techniques: Flow Cytometry, Clone Assay

Journal: Acta Pharmaceutica Sinica. B

Article Title: Novel Pt(IV) complex OAP2 induces STING activation and pyroptosis via mitochondrial membrane remodeling for synergistic chemo-immunotherapy

doi: 10.1016/j.apsb.2023.11.032

Figure Lengend Snippet: The immune activation effect of OAP2 in vivo . (A–B) Analysis of mature DC cells in mouse spleen. Expression of CD80 and CD86 quantitatively detected by flow cytometry (A), and statistical analysis (B). (C–D) Analysis of mature DC cells in mouse tumor site. Expression of CD80 and CD86 quantitatively detected by flow cytometry (C), and statistical analysis (D). (E–F) Analysis of cytotoxic T cells in mouse tumor. Expression of CD4 and CD8 quantitatively detected by flow cytometry (E), and statistical analysis (F). (G–N) Tumor tissue cytokine expression content measured by ELISA ( n = 3).

Article Snippet: The following antibodies and kits were used in the flow cytometry and ELISA analyses: CoraLite® Plus 488 Anti-Mouse CD4, CoraLite®568 Anti-Mouse CD3, APC Anti-Mouse CD86, FITC Plus Anti-Mouse CD11c, and PE Anti-Mouse CD80 (B7-1) were purchased from Proteintech.

Techniques: Activation Assay, In Vivo, Expressing, Flow Cytometry, Enzyme-linked Immunosorbent Assay